ABSTRACT
Objectives To determine the association between serum levels of high-mobility group box-1 (HMGB1),insulin-like growth factor-1 (IGF-1),vascular endothelial growth factor 165 (VEGF165) and occurrence and development of diabetic nephropathy (DN).Methods A total of 136 patients diagnosed as diabetic nephropathy (DN group) in Huai'an First People's Hospital between January 2016 to January 2018 were randomly selected in the study,including microalbuminuria group (n=62),macroalbuminuria group (n=50) and renal insufficiency group (n=24).Meanwhile,115 healthy examiners during the same period were collected as normal control group.Serum glucose,serum total cholesterol (TC),serum triglyceride (TG),high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C) and urinary albumin/urine creatinine ratio (UAlb/Cr) were detected inall subjects.Enzyme-linked immunosorbent assay (ELISA) was adopted to detect the serum concentrations of HMGB1,IGF-1 and VEGF165.Pearson correlation test was used to analyze the correlation between serum HMGB1,IGF-1 and VEGF165.Logistic ordered multi-classification regression was used to analyze the risk factors of DN progression,and the receiver operating characteristic curve (ROC) was drawn to evaluate the clinical predictive value of HMGB1,IGF-1 and VEGF165 in the progression of DN.Results The concentrations of serum HMGB1,IGF-1 and VEGF165 in DN patients were significantly higher than those in the control group (all P ＜ 0.05).There was a positive association between HMGB1 and IGF-1,HMGB1 and VEGF165,IGF-1 and VEGF165 (all P ＜ 0.01).Logistic regression analysis showed that elevated levels of HMGB1,IGF-1 and VEGF165 were independent risk factors for DN progression (OR=5.50,1.05,1.24,all P ＜ 0.05).The sensitivity,specificity and area under ROC curve of combined detection of HMGB1,IGF-1 and VEGF165 were higher than HMGB1,IGF-1 and VEGF165 alone (AUC=0.989,0.984,0.942,0.878,P ＜ 0.05).Conclusions The serum levels of HMGB1,IGF-1 and VEGF165 are related to the severity of DN.The clinical predictive value of combined detection of HMGB1,IGF-1 and VEGF165 for DN progression is superior to that of single index detection of HMGB1,IGF-1 and VEGF165.
ABSTRACT
To determine the associations between expressions of insulin-like growth factor-Ⅰ( IGF-Ⅰ), tumor necrosis factor-α (TNF-α), and vascular endothelial growth factor (VEGF) 165 in serum, and the occurrence and development of diabetic retinopathy ( DR). A total of 129 patients diagnosed as DM in our hospital between September 2015 and September 2017 were randomly selected in the study, including 58 cases single DM, diabetes with non-proliferative retinopathy group ( 45 cases) and diabetic proliferative retinopathy group ( 26 cases). Meanwhile, 110 healthy examinees during the same period were collected as a normal control group. The severity of retinopathy was judged by fundus photography and fundus fluorescein angiography. Blood glucose, BMI, HbA1C , TC, TG, HDL-C, LDL-C, and VLDL-C were detected in all subjects. Enzyme-linked immunosorbent assay (ELISA) was adopted to detect the serum concentrations of IGF-Ⅰ, TNF-α, and VEGF165. The expressions of serum IGF-Ⅰ, TNF-α, and VEGF in DM patients were significantly higher than those in the control group (all P<0.05). As diabetic retinopathy worsened, the expressions of serum IGF-Ⅰ, TNF-α, and VEGF increased. Pearson correlation analysis showed that serum IGF-Ⅰ was positively correlated with TNF-α, TNF-α, and VEGF165, IGF-Ⅰ, and VEGF165(P<0.05). Logistic regression analysis showed that IGF-Ⅰ and VEGF165 were risk factors for DR (OR = 1.059, 1.165, all P<0.05). The sensitivity, specificity and diagnostic accuracy of IGF-Ⅰ, TNF-α, and VEGF165 were higher than IGF-Ⅰ, TNF-α, and VEGF165 alone(AUC = 0.968, 0.928, 0.792, 0.893, all P<0.05). The expression level of IGF-Ⅰ, TNF-α, and VEGF165 in serum was related to the severity of diabetic retinopathy. The diagnosis of diabetic retinopathy with combined IGF-Ⅰ, TNF-α, and VEGF165 measurements was better than the single index of IGF-Ⅰ, TNF-α, and VEGF165.
ABSTRACT
Objective To investigate the impact of uric acid on peripheral blood glucose measured by the portable blood glu‐cose meter .Methods A total of 60 inpatients with 2 diabetic mellitus(T2DM ) complicating hyperuricemia were chosen as the re‐search group and contemporaneous 60 cases of T2DM without complicating hyperuricemia as the control group .Blood glucose in both groups was measured by the portable blood glucose meter ,meanwhile plasma glucose and serum uric acid were assessed by the biochemical analyzer in order to explore the impact of uric acid on peripheral blood glucose .Results (1)The difference between pe‐ripheral blood glucose and venous blood glucose was correlated with serum uric acid .(2)There was statistically significant differ‐ence in the blood glucose between the two groups .(3 ) The bias of blood glucose value were within the acceptable range . Conclusion Serum uric acid has certain influence on the peripheral blood glucose ,but which is within the acceptable range .The de‐tected blood glucose value of the portable blood glucose meter can be trusted .
ABSTRACT
Objective To investigate and analyze the dwarf reasons for children in the area of Huai'an city,Jiangsu province.Methods A retrospective analysis of 92 cases of children with short stature in our hospital in recent 5 years had been made.Results The dwarf reasons for the 92 cases of undersized children were:lack of growth hormone (53.3％),physical sexual puberty delay (16.3％),hypothyroidism (9.8％),turner syndrome (7.6％),nanosoma essentialis (5.4％),familial short stature (4.3％),intrauterine growth retardation (2.2％)and glycogen storage disease type Ⅰ (1.1％).Conclusion The main dwarf reasons for children were growth hormone deficiency and physical sexual puberty delay,and medical treatment should be used as soon as possible.